| Sprache / Charakter |
 |
 |
| Ezio Auditore | Markus Pfeiffer | Roger Craig Smith |
| Lucy Stillman | Susanne Dobruskin | Kristen Bell |
| Desmond Miles | Thomas Friebe | Nolan North |
| Mario Auditore | Helmut Krauss | Fred Tatasciore |
| Leonardo Da Vinci | Simon Jäger | Carlos Ferro |
| Rodrigo Borgia | Volker Wolf | Manuel Tadros |
| Giovanni Auditore | Tom Vogt | Romano Orzari |
| Maria Auditore | Edda Fischer | Ellen David |
| Claudia Auditore | Susanne Dobrusskin | Angela Gallupo |
| Frederico Auditore | Lars Schmidtke | Elias Toufexis |
| Antonio de Magianis | Carlo Mestroni | |
| Rosa | Ilya Welter | Lita Tresierra |
| Uberto Alberti | Hans Bayer | Michel Perron |
| Carlo Grimaldi | Roc Lafortune | |
| Lorenzo de Medici | Alex Ivanovici | |
| Bartolomeo D'Alviano | Alex Ivanovici | |
| Jacopo de Pazzi | Arthur Grosser | |
| Emilio Barbarigo | Hans Bayer | Arthur Holden |
| Shaun Hastings | Philipp Schepmann | Danny Wallace |
| Rebecca Crane | Michaela Breit | Eliza Jane Schneider |
| Subject 16 | Fritz Rott | Cam Clarke |
| Silvio Barbarigo Il Rosso | Hans-Gerd Kilbinger | Harry Stanjovski |
| Marco Barbarigo | Tony Robinow | |
| La Volpe | Stefan Margenfeld | Vito de Filippo |
| Vieri de Pazzi | YuriLowenthal | |
| Francesco de Pazzi | Thomas Balou-Martin | Andreas Apergis |
| Paola | Claudia Ferri | |
| Minerva | Margaret Easley | |
| Teodora | Nadia Verucci | |
| Annetta | Vanessa Wunsch | Anne Marie Baron |
| Caterina Sforza | Cristina Rosato | |
| Machiavelli | Shawn Baichoo | |
| Silvio Barbarigo | Hans-Gerd Kilbinger | Harry Standjofski |
| Doge Giovanni Mocenigo | Helmut Krauss | Ivan Freud |
| Agostino Barbarigo | Uli Krohm | |
| Christina Verpucci | Britt Gülland | Amber Goldfarb |
| Petruccio Auditore | Susanne Dobrusskin | |
| Ugo | Philipp Schepmann | Elias Toufexis |
| Warren Vidic | Rainer Delventhal | Phil Proctor |
