| Sprache / Charakter |
 |
 |
| Ezio Auditore | Markus Pfeiffer | Roger Craig Smith |
| Altair Ibn La-Ahad | Markus Pfeiffer | Cas Anvar |
| Desmond Miles | Thomas Friebe | Nolan North |
| Adam - DLC3 | Volker Niederfahrenhorst | Nolan North |
| William Miles | Bodo Wolf | John de Lancie |
| Shaun Hastings | Philipp Schepmann | Danny Wallace |
| Rebecca Crane | Michaela Breit | Eliza Jane Schneider |
| Warren Vidic | Rainer Delventhal | Phil Proctor |
| Darim Ibn La-Ahad / Abbas Hauptmänner / Masyaf Assassinen | Charles Rettinghaus | Michael Benyaer |
| Laetitia England | Amy Landecker | |
| Shahkulu | Tony Calabretta | |
| Clay Kaczmarek (Subjekt 16) | Fritz Rott | Graham Cuthbertson |
| Lysistrata / Mirela Djuric / Eve - DLC 3 | Maria Koschny | Ida Darvish |
| Abbas Sofian / Masyaf Assassinen | Tim Möseritz | Yermar Gur |
| Ahmet | Erich Räuker | Tamer Hassan |
| Piri Reis | Bert Franzke | Alex Ivanovici |
| Leandros | Ronald Nitschke | Steve Blum |
| Maria Thrope | Elisabeth Grünwald | Eleanor Noble |
| Yusuf Tarzim | Jan Kurbjuweit | Chris Parson |
| Juno | Claudia Gahrke | Nadia Verrucci |
| Jupiter | Ernst Meincke | Peter Renaday |
| Al Mualim | Erich Räuker | Peter Renaday |
| Animus | Heike Hagen | Jenifer Seguin |
| Suleiman I | Nico Sablik | Haaz Sleiman |
| Sofia Sator | Claudia Urbschat-Mingues | Carlotta Montanari |
| Manuel Palaiologos | Jürgen Kluckert | Vlasta Vrana |
| Leandros | Ronald Nitschket | Steve Blum |
