| Sprache / Charakter |
 |
 |
| Ezio Auditore | Markus Pfeiffer | Roger Craig Smith |
| Lucy Stillman | Susanne Dobrusskin | Kristen Bell |
| Desmond Miles | Thomas Friebe | Nolan North |
| Mario Auditore | Helmut Krauss | Fred Tatasciore |
| Leonardo Da Vinci | Simon Jäger | Carlos Ferro |
| Rodrigo Borgia | Volker Wolf | Manuel Tadros |
| Claudia Auditore | Susanne Dobrusskin | Angela Gallupo |
| Frederico Auditore | Lars Schmidtke | Elias Toufexis |
| Bartolomeo D'Alviano | Alex Ivanovici | |
| Octavien | Arthur Holden | |
| Lucrezia Borgia | Liane Balaban | |
| Shaun Hastings | Philipp Schepmann | Danny Wallace |
| Rebecca Crane | Michaela Breit | Eliza Jane Schneider |
| Juan Borgia the Elder | Harry Stanjovski | |
| La Volpe | Stefan Margenfeld | Vito de Filippo |
| Cesare Borgia | Andreas Apergis | |
| Animus | Jenifer Seguin | |
| Caterina Sforza | Cristina Rosato | |
| Machiavelli | Shawn Baichoo |
